When attempting to run a crispritz search on a non-human primate genome, using the following command:
'python ~/miniconda3/pkgs/crispritz-2.6.6-py39h243b37b_0/bin/crispritz.py search Mnem_1.0/ 20bp-NGG-SpCas9.txt gla-NM-gRNA-1.txt gla.Mnem1 -mm 4 -t -scores Mnem_1.0/'
I get the following error::
'Search START USED THREADS 8 ANALYZING CHROMOSOME NW_012020308.1 Macaca nemestrina isolate M95218 unplaced genomic scaffold, Mnem_1.0 Scaffold999, whole genome shotgun sequence (Total progress: 100.0%) terminate called after throwing an instance of 'std::length_error' what(): vector::_M_default_append Search END Search runtime: 24.96618342399597 seconds Traceback (most recent call last): File "/home/va/miniconda3/pkgs/crispritz-2.6.6-py39h243b37b_0/opt/crispritz/Python_Scripts/Scores/scores.py", line 211, in save_targ_scor.write(next(result).strip() + '\tCFD\n') # Add header StopIteration `
Genome is unindexed since we were not planning on doing a bulge search, at least not initially. The gRNA and Cas9 text files are attached.
Software versions:
WSL2 on Windows 11 with Ubuntu 20.04
Python 3.9
gla-NM-gRNA-1.txt
20bp-NGG-SpCas9.txt
When attempting to run a crispritz search on a non-human primate genome, using the following command:
'python ~/miniconda3/pkgs/crispritz-2.6.6-py39h243b37b_0/bin/crispritz.py search Mnem_1.0/ 20bp-NGG-SpCas9.txt gla-NM-gRNA-1.txt gla.Mnem1 -mm 4 -t -scores Mnem_1.0/'
I get the following error::
'Search START USED THREADS 8 ANALYZING CHROMOSOME NW_012020308.1 Macaca nemestrina isolate M95218 unplaced genomic scaffold, Mnem_1.0 Scaffold999, whole genome shotgun sequence (Total progress: 100.0%) terminate called after throwing an instance of 'std::length_error' what(): vector::_M_default_append Search END Search runtime: 24.96618342399597 seconds Traceback (most recent call last): File "/home/va/miniconda3/pkgs/crispritz-2.6.6-py39h243b37b_0/opt/crispritz/Python_Scripts/Scores/scores.py", line 211, in save_targ_scor.write(next(result).strip() + '\tCFD\n') # Add header StopIteration `
Genome is unindexed since we were not planning on doing a bulge search, at least not initially. The gRNA and Cas9 text files are attached.
Software versions:
WSL2 on Windows 11 with Ubuntu 20.04
Python 3.9
gla-NM-gRNA-1.txt
20bp-NGG-SpCas9.txt